Sexual and reproductive health

WHO laboratory manual for the examination and processing of human semen

Fifth edition

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Authors:
World Health Organization, Department of Reproductive Health and Research

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Publication details

Number of pages: 287
Publication date: 2010
Languages: Chinese, English, Italian, Japanese
Price: CHF 50.00 / US$ 60.00 - Developing countries: CHF 35.00
ISBN: 978 92 4 154778 9

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Semen analysis may be useful in both clinical and research settings, for investigating male fertility status as well as monitoring spermatogenesis during and following male fertility regulation and other interventions. This manual provides updated, standardized, evidence-based procedures and recommendations for laboratory managers, scientists and technicians to follow in examining human semen in a clinical or research setting. Detailed protocols for routine, optional and research tests are elaborated.

The fifth edition includes new information on sperm preparation for clinical use or specialized assays and on cyropreservation, an expanded section on quality control in the semen analysis laboratory and evidence-based reference ranges and reference limits for various semen characteristics. The methods described are intended to improve the quality of semen analysis and the comparability of results from different laboratories.