Test procedures for insecticide resistance monitoring in malaria vector mosquitoes

April 2013


World Health Organization

Publication details

Number of pages: 39
Publication date: 2013
Languages: English
ISBN: 9789241505154



The Global plan for insecticide resistance management in malaria vectors (GPIRM) – released in May 2012 – highlights the importance of routinely collecting, analyzing, managing and sharing data on insecticide resistance. This publication:

  • provides background information on how the WHO susceptibility test has evolved leading to the current revision ;
  • focusing on adult mosquito testing, it provides details on discriminating concentrations of the insecticides to be tested, as well as recommended equipment and supplies, sampling protocols, test conditions and protocols;
  • explains how susceptibility test results are recorded and reported, i.e. calculation of mortality and knock-down rates, interpretation of susceptibility test results, reporting results of susceptibility testing;
  • where appropriate, recommends further investigations i.e. identification of resistance mechanisms such as biochemical enzyme assays, molecular (biological) tests and synergist assays;
  • for the first time the CDC bottle assay, an alternative susceptibility test - has been included in the test procedures.

One of the most interesting features of this publication is on the reporting of test results in line with GPIRM recommendations for pre-emptive action in managing insecticide resistance.