Influenza

WHO information for molecular diagnosis of influenza virus - update

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Publication details

Number of pages: 56
Publication date: March 2014
Languages: English

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This document provides information on the following molecular diagnostic protocols updated as of the above date for surveillance of influenza viruses in humans.

This is a revision of the document published on the WHO website in November 2012 with updated molecular protocols. The protocols are in the annexes below:

Annex 1: Conventional RT-PCR protocols (Page 6)
  • Conventional RT-PCR analyses for the matrix gene of influenza type A viruses (Page 6)
  • Conventional one step RT-PCR for A(H1N1)2009 HA gene (Page 9)
  • Conventional one step RT-PCR for A(H5N1) HA gene (Page 11)
  • Influenza B lineage specific one step conventional RT-PCR protocol (Page 14)
  • Conventional RT-PCR assays for the detection of influenza A(H1N1, H3N2), influenza B and influenza A(H5N1) viruses (Page 17)
Annex 2: Realtime RT-PCR protocols (Page 22)
  • Realtime RT-PCR test for the matrix gene of influenza type A viruses (Page 22)
  • One step realtime RT-PCR for H1 gene of A(H1N1)2009 virus (Page 24)
  • Influenza B lineage specific one-step realtime RT-PCR (Page 27)
  • Realtime RT-PCR assays for the detection of seasonal influenza viruses and influenza A(H5N1) viruses (Page 30)
  • One step realtime RT-PCR assays for human influenza A(H7N9) virus (Page 43)
  • Realtime RT-PCR assays for human influenza A(H10N8) virus (Page 50)
Annex 3: Sequencing protocols (Page 54)


Influenza A(H7N9)

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