Influenza

WHO information for molecular diagnosis of influenza virus in humans - update

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Publication details

Number of pages: 37
Publication date: November 2012
Languages: English

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This document provides information on the following molecular diagnostic protocols available as of the above date for influenza viruses in humans.

Annex 1: Conventional RT-PCR protocols (Page 5)
  • A. Conventional RT-PCR analyses for the matrix gene of influenza type A viruses (Page 5)
  • B. Conventional one step RT-PCR for A(H1N1)2009 HA gene (Page 8)
  • C. Conventional one step RT-PCR for A(H5N1) HA gene (Page 10)
  • D. Influenza B lineage specific one step conventional RT-PCR protocol (Page 13)
  • E. Conventional RT-PCR assays for the detection of influenza A(H1N1, H3N2), influenza B and influenza A(H5N1) viruses (Page 16)
Annex 2: Realtime RT-PCR protocols (Page 21)
  • A. Realtime RT-PCR test for the matrix gene of influenza type A viruses (Page 21)
  • B. One step realtime RT-PCR for H1 gene of A(H1N1)2009 virus (Page 23)
  • C. Influenza B lineage specific one-step realtime RT-PCR (Page 26)
  • D. Realtime RT-PCR assays for the detection of seasonal influenza viruses and influenza A(H5N1) viruses (Page 29)

Influenza A(H7N9)

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