Influenza

WHO information for molecular diagnosis of influenza virus - update

Publication details

Number of pages: 60
Publication date: July 2017
Languages: English

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This document provides information on the following molecular diagnostic protocols updated as of the above date for surveillance of influenza viruses in humans.

This is a revision of the document published on the WHO website in November 2012 with updated molecular protocols. The protocols are in the annexes below:

Annex 1: Conventional RT-PCR protocols (Page 8)
  • Conventional RT-PCR assays for the matrix gene of influenza type A viruses (Page 8)
  • Conventional one step RT-PCR for A(H1N1)pdm09 HA gene (Page 11)
  • Conventional one step RT-PCR for A(H5N1) (Page 13-15)
  • Influenza B lineage specific one step conventional RT-PCR protocol (Page 16)
  • Conventional RT-PCR assays for the detection of influenza A(H1N1, H3N2), influenza B (Page 19)
Annex 2: Realtime RT-PCR protocols (Page 22)
  • Realtime RT-PCR test for the matrix gene of influenza types A, B and C viruses (Page 23)
  • One step realtime RT-PCR for H1 gene of A(H1N1)2009 virus and A(H3N2) viruses (Page 26-28)
  • Influenza B lineage specific one-step realtime RT-PCR (Page 29)
  • Realtime RT-PCR assays for the detection of seasonal influenza viruses, A(H5N1), A(H7N9) and A(H9N2) viruses (Page 30-44)
  • One step realtime RT-PCR assays for human influenza A(H7) virus (Page 46-51)
  • Realtime RT-PCR assays for human influenza A(H10N8) virus (Page 55)
Annex 3: Sequencing protocols for influenza A(H7N9)(Page 58)


For CDC RT-PCR protocols, please follow this link

Influenza A(H7N9)

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