Buruli ulcer

Buruli ulcer - Diagnosis of Mycobacterium ulcerans disease

WARNING - disturbing photos

Ulcerative forms

Bone involvement
Hand (Fig. 6a)
Leg (Fig. 6b)
Back (Fig. 6c)
Forearm (Fig. 6d)

Bone involvement

Osteomyelitis (Fig. 7)

Sequelae

Hypertrophic scar (Fig. 9)
Squamous cell carcinoma (Fig. 10)
Figure 10

Differential diagnosis

Leishmaniasis (Fig. 11a)
Tropical phagedenic ulcer (Fig. 11b)
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Table of content

  1. Preface
  2. Summary
  3. Introduction
  4. Clinical diagnosis
  5. Biosafety and record-keeping in the laboratory
  6. Collection and transport of clinical specimens
  7. Secondary bacterial infection in M. ulcerans disease
  8. Microbiological methods for diagnosis
  9. Histopathological methods for diagnosis
  10. Annex 1 - Flow-chart for the laboratory diagnosis of Mycobacterium ulcerans disease
  11. Annex 2 - Laboratory request form
  12. Annex 3: Laboratory report form
  13. Annex 4 - Preparation of culture media
  14. Annex 5 - Microbiological staining techniques
  15. Annex 6 - Histopathological staining techniques
  16. Annex 7 - Decontamination methods
  17. Annex 8 - Mycobacterium ulcerans culture with BACTEC 460 TB instrument
  18. Annex 9 - Biochemical and culture tests used for the identification of slow-growing mycobacteria
  19. Annex 10 - Polymerase chain reaction (PCR) protocol
  20. Annex 11 - Manufacturers' addresses
  21. Annex 12 - Work of WHO on Buruli ulcer
  22. Annex 13 - Some research institutions involved in Buruli ulcer activities
  23. Annex 14 - Some nongovernmental organizations and others involved in Buruli ulcer activities
  24. Suggested reading
  25. Acknowledgements and contributors
  26. Illustrations - Credits
  27. WARNING - disturbing photos