Provider-initiated symptom screening for tuberculosis in Zimbabwe: diagnostic value and the effect of HIV status
Elizabeth L Corbett a, Abbas Zezai b, Yin Bun Cheung c, Tsitsi Bandason b, Ethel Dauya b, Shungu S Munyati b, Anthony E Butterworth a, Simba Rusikaniko d, Gavin J Churchyard e, Stanley Mungofa f, Richard J Hayes c & Peter R Mason b
a. Clinical Research Unit, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, England.
b. Biomedical Research and Training Institute, Harare, Zimbabwe.
c. Infectious Disease Epidemiology Unit, London School of Hygiene and Tropical Medicine, London, England.
d. University of Zimbabwe College of Health Sciences, Harare, Zimbabwe.
e. Aurum Institute, Johannesburg, South Africa.
f. Harare City Health, Harare, Zimbabwe.
Correspondence to Elizabeth L Corbett (e-mail: email@example.com).
(Submitted: 07 August 2008 – Revised version received: 18 February 2009 – Accepted: 05 March 2009.)
Bulletin of the World Health Organization 2010;88:13-21. doi: 10.2471/BLT.08.055467
Symptom questionnaires provide a quick, cheap and convenient way to identify individuals at a high risk of tuberculosis (TB) disease, termed TB suspects, who then need investigation with more definitive tests such as sputum microscopy, chest radiography and, when available, TB culture. Symptom screening for TB has high sensitivity when used to define TB suspects among patients who present themselves to health-care facilities for investigation of ill-health (i.e. passive case-finding) and is a key component of the the World Health Organization’s DOTS strategy for combating TB. In particular, chronic cough was found to be both highly sensitive and to have a reasonably high positive predictive value for smear-positive TB at the primary health-care level in studies in the pre-HIV era leading up to the development of the DOTS strategy.1–3
More recently, provider-initiated TB screening (i.e. active case-finding) has become an important part of HIV care in resource-poor settings.4–8 Ruling out active TB is necessary both for individual patient management and for TB infection control. Symptom screening is often the only practical approach but, at least in HIV-negative (HIV−) individuals, TB symptom screening is considerably less sensitive when used for provider-initiated TB screening than when applied to patients who have self-presented for the investigation of ill health.9–15 Evidence is mounting that this may also be true for HIV-positive (HIV+) individuals.5–8,12–16 Screening failure can have major adverse consequences. In patients starting antiretroviral therapy, undiagnosed TB is common and carries a substantially increased risk of death or hospitalization in the first few months of treatment.8,17 Institutional outbreaks of TB have caused high morbidity and mortality among HIV-infected outpatients, especially in South Africa.18 Perhaps most seriously, isoniazid preventive therapy is rarely used in Africa, despite its proven effectiveness for preventing HIV-related TB: fear of the consequences of screening failure and the subsequent risk of generating drug resistance is one of the major reasons for this implementation failure.19
In this study, we evaluated different approaches to TB screening in participants of a population-based HIV and TB prevalence survey in Harare, Zimbabwe. A TB symptom questionnaire was administered, and all participants were screened by sputum culture (even if asymptomatic) and followed-up to confirm or exclude TB. The main aim of this study was to investigate the effect of HIV on the prevalence of TB symptoms and on the diagnostic utility of different screening strategies among survey participants not being treated for TB.
Participants and setting
Details of recruitment into the prevalence survey have been described more fully elsewhere.20 Briefly, a population-based survey was carried out in 2005–2006 among a random sample of adults in 46 previously enumerated neighbourhoods in the high-density suburbs of Harare. Participants were asked to answer a questionnaire, provide blood for anonymized HIV serum testing and provide two sputum specimens (one “spot” specimen and one early morning specimen) for TB culture. Voluntary HIV counselling and testing was provided separately through outreach clinics by the New Start Centre, Harare, Zimbabwe.
Definition and follow-up of TB suspects
A TB suspect was defined as an individual with any TB symptom (defined below) or who tested positive on sputum culture during screening (i.e. culture-positive). Consequently, asymptomatic culture-negative TB would not have been detected. The TB symptoms considered were: current cough of any duration or severity, haemoptysis during the previous year, self-reported fever or “hot body”, night sweats and a subjective report of weight loss. Individuals who reported night sweats were asked whether they had to change their bed clothes or sheets (i.e. drenching night sweats) or not (i.e. mild night sweats). An enquiry was made about the duration (in weeks) of any reported symptoms. Cough was defined as acute if less than 2 weeks’ duration and as chronic otherwise. Smoking history and alcohol intake, assessed using the Alcohol Use Disorders Identification Test questionnaire,21 were also recorded.
In addition, TB suspects underwent follow-up testing, which included repeat sputum microscopy and culture and chest radiography, until TB was confirmed or excluded. A standard algorithm was used to investigate possible cases of culture-negative TB: it included analysing clinical and radiological responses to broad-spectrum antibiotics and the response to TB treatment at 1 month, where applicable.
Case definitions for TB disease
A case of TB disease was not diagnosed on the basis of screening results alone and subsequent evidence was required. One of the following case definitions had to be satisfied:
- definite TB: sputum culture-positive for Mycobacterium tuberculosis on two or more occasions (e.g. at screening and the first follow-up test);
- probable culture-positive TB: culture-positive for M. tuberculosis on one occasion (e.g. at screening or a follow-up test) plus the presence of a compatible clinical illness or radiological evidence of TB, failure to respond to broad-spectrum antibiotics, and a response to TB treatment at 1 month;
- probable culture-negative TB: culture-negative for M. tuberculosis plus radiological evidence of TB or the presence of progressive symptoms of TB, failure to respond to antibiotics, and a response to TB treatment at 1 month.
For participants with one or more symptoms, the two sputum specimens were processed separately for culture and sputum smears were read immediately. For asymptomatic participants, a single pooled sputum specimen was cultured and the two smears were read only if the pooled culture tested positive (hence we can not comment on the sensitivity of screening smears). Smears were made from concentrated sputum decontaminated with 4% sodium hydroxide and read by fluorescence microscopy with auramine O. All positive and 10% of negative slides were re-read by a second reader. Each positive result was confirmed using Ziehl–Neelsen staining. Culture used Löwenstein-Jensen slopes, with the residual concentrate stored at −20 °C for re-culture in the event of contamination. Species were identified using colony morphology, growth at different temperatures and growth on para-nitrobenzoic acid Löwenstein-Jensen slopes. Non-tuberculous mycobacteria were sent to the South African Institute for Medical Research in Johannesburg for further identification.
Confidential HIV serum testing was carried out with the Determine assay (Abbott Diagnostics, Johannesburg, South Africa), with all positive and 10% of negative specimens confirmed by the Unigold test (Trinity Biotech, Dublin, Ireland). For participants who were not willing to provide serum, oral mucosal transudate was collected and tested using the Vironostika assay (Vironostika, BioMérieux, Marcy l’Etoile, France).
Approval was granted by the Ethics Committees of the Biomedical Research and Training Institute, Harare, Zimbabwe, the Medical Research Council of Zimbabwe, Harare, Zimbabwe, and the London School of Hygiene and Tropical Medicine, London, United Kingdom. Written informed consent was provided by all participants. Confidential HIV tests were carried out and the results were stored using dedicated laboratory numbers, with no other personal identifiers. Voluntary counselling and testing were offered to all participants.
Data were captured using EpiInfo 2003 (Centers for Disease Control and Prevention, Atlanta, GE, USA) and analysed with STATA 9.0 software (STATA Corporation, College Station, TX, USA).
The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of symptoms and TB culture findings were calculated using the set of TB case definitions listed above as the reference or gold standard diagnosis. The area under the receiver operating characteristic curve (AUC) was also estimated to provide a summary measure of diagnostic accuracy. A non-parametric test was used to compare different AUCs. Logistic regression analysis was used to investigate risk factors for chronic cough and, in separate analyses, the relationship between TB disease and TB symptoms. There was no significant clustering of TB disease in communities or households (only three households had multiple TB cases), so in the analysis in which TB disease was the outcome, we did not adjust for clustering effects. However, for the analysis of TB symptoms, we used robust, or sandwich, standard errors to allow for clustering.
Of the 10 076 participants who consented to TB screening in the parent study, 93 were already on TB treatment (including 9 who were still culture-positive) and 1004 declined HIV-testing, leaving 8979 participants for this analysis. The participants’ baseline characteristics are shown by HIV status in Table 1.
As shown in Table 2, all potential TB symptoms were significantly more common in the 1858 HIV+ than the 7121 HIV− participants, except for acute cough. Overall, 21.2% of HIV+ and 9.9% of HIV− participants had at least one symptom (P < 0.001). The most frequent symptoms were weight loss, in 4.9% of the total, and drenching night sweats, in 4.0%. Chronic cough lasting 2 weeks or more was reported by 3.7% of participants: by 8.2% of HIV+ and 2.5% of HIV− participants.
Newly diagnosed TB disease that met one of the above case definitions was observed in 79 participants, whose sputum culture and smear results and HIV status are shown in Table 3. Symptoms differed little by HIV status (Table 3). Overall, 47 (59%) had cough, which was of 2 weeks’ duration or longer in 37 (47%). The next most common symptoms were unintentional weight loss in 47% and drenching night sweats in 38%. In 18 patients (24% of HIV+ and 22% of HIV− participants with TB disease), TB was detected by screening culture alone as they denied having any symptoms at baseline. They included 8 participants who also had smear-positive disease: 4 HIV− and 4 HIV+.
Table 3. Prevalence of specific sputum culture and smear screening test results and TB symptoms in 79 study participants with active TB, by HIV status, Zimbabwe, 2005
Duration of symptoms
There was no significant difference in the reported duration of symptoms between participants with symptoms who had TB and those who did not, except that cough was significantly more prolonged in HIV− participants who also had TB (median: 4 weeks, interquartile range: 2–8 weeks) than in those whose cough was due to another cause (median: 2 weeks, interquartile range: 1–4 weeks; P = 0.003). In HIV+ participants, however, cough due to another cause was also prolonged (median: 3.5 weeks, interquartile range: 2–8 weeks) and not significantly different from cough associated with HIV-related TB (median: 4 weeks, interquartile range: 2–8 weeks; P = 0.36).
In general, TB patients with symptoms tended to be polysymptomatic. Multivariate logistic regression analysis was used to identify symptoms and other variables independently associated with TB disease (Table 4). Odds ratios (ORs) adjusted for other symptoms tended to be considerably lower than unadjusted ORs. For both HIV+ and HIV− TB, acute cough (adjusted OR: 4.9 for HIV− and 12.6 for HIV+ TB), chronic cough (adjusted OR: 19.4 for HIV− and 6.1 for HIV+ TB) and weight loss (adjusted OR: 4.0 for HIV− TB and 2.8 for HIV-related TB) remained independent predictors of TB disease after adjustment. In addition, the presence of drenching night sweats was an independent predictor of HIV+ TB (adjusted OR: 3.1, 95% confidence interval: 1.4–7.1) but not of HIV− TB (adjusted OR: 1.4, 95% confidence interval: 0.4–5.4). Neither recent household contact with TB nor past TB treatment was significantly associated with TB after adjustment for symptoms.
Table 4. ORs for TB disease among HIV− and HIV+ participants for different symptoms, recent household contact with TB and past TB treatment, as derived by unadjusted and adjusted multivariate logistic regression analysis, Zimbabwe, 2005
Measures of the utility of different screening strategies for TB disease in HIV− and HIV+ individuals are reported in Table 5. Although HIV status did not have a significant effect on either sensitivity or specificity, PPVs were considerably higher in HIV+ participants.
Table 5. Diagnostic value of different symptoms and other variables and their combination for screening for TB disease in HIV− and HIV+ individuals, Zimbabwe, 2005
No screening strategy was ideal: all missed some TB patients and the highest AUC for symptom-based screening was 0.82 for HIV− TB and 0.81 for HIV+ TB.
In a pooled analysis of HIV+ and HIV− TB suspects, prolonged cough had a sensitivity of 46.8% and a specificity of 96.7% for identifying TB disease. The sensitivity increased to 59.5% for cough of any duration, with a significant increase in AUC from 0.72 for chronic cough to 0.77 (P = 0.005). There was a further significant increase in AUC to 0.81 (P = 0.037 for the comparison with any cough) for weight loss or cough (sensitivity: 69.6%). The maximum sensitivity was 77.2% for the criterion of exhibiting any one or more of the five TB symptoms used to define a TB suspect. However, there was no significant increase in AUC compared with cough or weight loss because of falls in both specificity (from 91.7% for cough or weight loss to 88.3% for any symptom) and PPV (from 6.9% for cough or weight loss to 6.2% for any symptom).
The use of TB culture on solid culture media for screening for TB disease had a sensitivity of 68.4% and an AUC of 0.84. The specificity was just under 100% because M. tuberculosis grew on specimens from 16 participants, including 14 who were HIV−, in whom TB was not confirmed. Of these 16, 6 had TB symptoms, while follow-up was suboptimal in 4 (2 of whom were both symptomatic and HIV+) because they refused further investigation or relocated. The 12 participants who were followed up were known to have remained clinically stable for several months while off TB treatment. The prevalence of TB symptoms (37.5%) in culture-positive individuals in whom TB was not confirmed was significantly higher than in culture-negative individuals without TB (11.7%, P = 0.007). There was no other significant difference in patient characteristics between these two groups.
This study shows that some screening failures can be anticipated when either TB symptoms alone or M. tuberculosis culture alone is used to screen for previously undiagnosed TB, even in HIV-infected individuals. For example, M. tuberculosis grew on culture of sputum specimens from 19 HIV− and 9 HIV+ participants who did not exhibit any of the five TB symptoms considered (i.e. cough, haemoptysis, fever, night sweats or weight loss) and, subsequently, 9 of the 19 HIV− and all 9 HIV+ participants had TB disease confirmed on follow-up. In addition, screening cultures were negative in 25 patients in whom TB disease was confirmed either by positive cultures on follow-up (4 HIV+ and 5 HIV− participants) or from clinical and radiological responses to TB treatment (13 HIV+ and 3 HIV− participants).
The prevalence of TB symptoms was high in HIV+ participants and the sensitivity of initial symptom screening in these individuals ranged from 47.9% when chronic cough was used to define a TB suspect to 81.3% when any of the five TB symptoms considered was used. Symptom screening was more sensitive than sputum culture on a solid medium, whose sensitivity was 64.6%. We found that the diagnostic performance of symptom screening in HIV+ participants, as assessed using the AUC, was significantly and incrementally better when acute cough of less than 2 weeks’ duration and weight loss were individually added to the cardinal symptom of chronic cough lasting 2 weeks or more. Broadening the definition of a TB suspect further did not improve diagnostic performance. Nevertheless, PPVs were high for all symptoms among HIV+ participants, which supports using a less strict definition of a TB suspect in individuals known to have an HIV infection, even though specificities were suboptimal.
Although subclinical TB disease is rarely recognized in routine clinical practice, it is often observed during provider-initiated TB testing whenever a second screening modality (e.g. radiography or diagnostic bacteriology) is combined with symptom screening.7,9–16 Fig. 1 illustrates why the symptom and bacteriological profiles of TB patients identified through provider-initiated TB testing will tend to differ from those of patients diagnosed through routine TB clinical services, especially when a strong routine diagnosis and treatment programme is in place. In the latter case, patients with highly symptomatic disease and those with “first-line” diagnostic features, such as chronic cough or a positive smear test result, will tend to be rapidly diagnosed and treated, leaving behind a residual burden of TB patients with less clinically obvious TB and those who have not sought care. Moreover, TB patients with more severe symptoms are more likely to be smear-positive, as are those whose symptoms include chronic cough.22,23 These factors help reconcile observations from provider-initiated prevalence surveys carried out in the pre-TB treatment era, when chronic cough had a high sensitivity for TB,22 with observations from populations who now have access to TB diagnosis and treatment, in whom chronic cough is typically reported by only half or fewer of prevalent cases.7,9–16
Fig. 1. Effect of strong routine TB diagnosis and treatment programmesa on symptom and bacteriological profiles of residual TB cases that remain to be detected through provider-initiated TB screening
In extreme cases, provider-initiated surveys of TB in populations with very easy access to TB diagnosis and treatment have reported that all symptom combinations have very low sensitivity.12,14 This scenario should, in fact, be the aim of most HIV care clinics, where regular provider-initiated screening based on TB symptoms is now a World Health Organization recommendation.4 Such screening is a valuable component of individual patient management and TB infection control, but health-care providers working in these settings should not, then, be surprised by the resulting low sensitivity of TB symptoms for detecting undiagnosed TB.
In the community-based survey reported here, HIV infection was associated with a significantly increased burden of undiagnosed TB and also with a higher frequency of symptoms, including weight loss, fever, drenching night sweats and chronic cough. This higher frequency was due to increases in both TB and non-TB causes of symptoms, and so had little effect on the sensitivity or specificity of symptom screening. Neither recent household TB contact nor past TB treatment was an independent predictor of prevalent TB. Nor did these factors lead to an increase in the yield of TB detected, though their presence substantially increased the number of defined TB suspects and the cost of subsequent investigation. The value of including past TB treatment as a “screening” question may be higher in some populations, in which up to half of prevalent TB cases report past TB treatment,24 but our results suggest that including recent household TB contact is likely to be expensive and uninformative.
One major and important difference between HIV+ and HIV− participants was that the duration of cough had limited diagnostic utility in HIV+ participants, with both acute and chronic cough having similar odds ratios for TB. In contrast, in HIV− participants, the odds ratio for chronic cough was much higher than for acute cough. This difference was mainly due to chronic cough being common in HIV+ participants without TB. Acute cough, with or without radiological changes, has been reported to have a relatively high PPV for TB in both provider-initiated screening6 and in self-presenting patients in areas where HIV is prevalent,25–28 and so should ideally be considered as a TB symptom in both these contexts.
The limitations of this survey are that the questionnaire used included a limited range of questions, chest radiography was not included as a third screening modality and participants were not examined for signs of extrapulmonary TB. Consequently, we will have missed some cases of asymptomatic or minimally symptomatic, culture-negative TB.6,11,14 In addition, we used solid media for TB culture, which have a lower sensitivity than some liquid culture systems such as the Mycobacterial Growth Indicator Tube,29 and we only examined sputum smears in symptomatic or culture-positive patients. Since our prevalence survey was population-based, the HIV+ participants included probably had different patient characteristics and a lower burden of undiagnosed TB than participants in surveys based in HIV clinics. We did not investigate the possibility that antiretroviral use modified the burden of TB in HIV-infected participants, as information on HIV status and access to HIV care were both limited at the time of this survey. At baseline, we excluded nine culture-positive cases who were recently diagnosed with TB: including them would have increased the proportion of TB patients with chronic cough from 47% to 51%.
In summary, we have shown that even smear-positive TB may be missed by symptom screening in HIV+ TB patients. Although the sensitivity and specificity of symptom screening were similar for HIV+ and HIV− participants, the presence of symptoms in HIV+ participants had a higher PPV and a lower NPV. Although imperfect, three of the symptoms evaluated (i.e. cough, drenching night sweats and weight loss) were independently predictive of TB and their combination had a NPV over 99% in HIV+ participants. This suggests that symptom screening alone may be able to identify, at least in some settings, a subset of patients who are at a low risk of undiagnosed TB and in whom antiretroviral therapy or isoniazid preventive therapy can be started without further screening. This suggestion would, however, need confirmation from prospective studies ideally conducted in settings with both high and low prevalences of TB, given the limitations of the current study design. Provider-initiated TB screening is one of the many aspects of TB–HIV management in urgent need of more effective and accessible TB diagnostic tools. Unnecessary TB investigations are costly for patient and provider alike, do not always result in diagnostic certainty and delay access to life-saving antiretroviral therapy and preventive TB treatment. Consequently until better TB diagnostics for resource-poor settings become widely available, many providers of HIV care in Africa will remain in the unsatisfactory position of having to weigh the adverse consequences of potentially avoidable screening failures against those of setting unrealistically high screening standards. ■
We thank the survey participants and all staff members of the Biomedical Research and Training Institute, Harare, Zimbabwe for their contribution to this study. The study was funded by the Wellcome Trust, London, United Kingdom. Additional author affiliations are: Biomedical Research and Training Institute, Harare, Zimbabwe (ELC, AEB), National Institute of Health Research, Harare, Zimbabwe (SSM) and the University of Zimbabwe College of Health Sciences, Harare, Zimbabwe (PRM).
Competing interests: None declared.
- Aluoch JA, Swai OB, Edwards EA, Stott H, Darbyshire JH, Fox W, et al., et al. Study of case-finding for pulmonary tuberculosis in outpatients complaining of a chronic cough at a district hospital in Kenya. Am Rev Respir Dis 1984; 129: 915-20 pmid: 6732051.
- Fox W. Tuberculosis case-finding and treatment programmes in the developing countries. Br Med Bull 1988; 44: 717-37 pmid: 3076817.
- Raviglione MC, Pio A. Evolution of WHO policies for tuberculosis control, 1948-2001. Lancet 2002; 359: 775-80 doi: 10.1016/S0140-6736(02)07880-7 pmid: 11888605.
- World Health Organization. Interim policy on collaborative TB/HIV activities. Geneva: World Health Organization; 2004 (WHO/HTM/TB/2004.33).
- Mohammed A, Ehrlich R, Wood R, Cilliers F, Maartens G. Screening for tuberculosis in adults with advanced HIV infection prior to preventive therapy. Int J Tuberc Lung Dis 2004; 8: 792-5 pmid: 15182152.
- Day JH, Charalambous S, Fielding KL, Hayes RJ, Churchyard GJ, Grant AD. Screening for tuberculosis prior to isoniazid preventive therapy among HIV-infected gold miners in South Africa. Int J Tuberc Lung Dis 2006; 10: 523-9 pmid: 16704034.
- Lawn SD, Myer L, Bekker LG, Wood R. Burden of tuberculosis in an antiretroviral treatment programme in sub-Saharan Africa: impact on treatment outcomes and implications for tuberculosis control. AIDS 2006; 20: 1605-12 doi: 10.1097/01.aids.0000238406.93249.cd pmid: 16868441.
- Bonnet MM, Pinoges LL, Varaine FF, Oberhauser BB, O’Brien DD, Kebede YY, et al., et al. Tuberculosis after HAART initiation in HIV-positive patients from five countries with a high tuberculosis burden. AIDS 2006; 20: 1275-9 doi: 10.1097/01.aids.0000232235.26630.ee pmid: 16816556.
- Tupasi TE, Radhakrishna S, Rivera AB, Pascual ML, Quelapio MI, Co VM, et al., et al. The 1997 nationwide tuberculosis prevalence survey in the Philippines. Int J Tuberc Lung Dis 1999; 3: 471-7 pmid: 10383058.
- Hong YP, Kim SJ, Lew WJ, Lee EK, Han YC. The seventh nationwide tuberculosis prevalence survey in Korea, 1995. Int J Tuberc Lung Dis 1998; 2: 27-36 pmid: 9562108.
- den Boon S, White NW, van Lill SW, Borgdorff MW, Verver S, Lombard CJ, et al., et al. An evaluation of symptom and chest radiographic screening in tuberculosis prevalence surveys. Int J Tuberc Lung Dis 2006; 10: 876-82 pmid: 16898372.
- Corbett EL, Charalambous S, Moloi VM, Fielding K, Grant AD, Dye C, et al., et al. Human immunodeficiency virus and the prevalence of undiagnosed tuberculosis in African gold miners. Am J Respir Crit Care Med 2004; 170: 673-9 doi: 10.1164/rccm.200405-590OC pmid: 15191919.
- Corbett EL, Bandason T, Cheung YB, Munyati S, Godfrey-Faussett P, Hayes R, et al., et al. Epidemiology of tuberculosis in a high HIV prevalence population provided with enhanced diagnosis of symptomatic disease. PLoS Med 2007; 4: e22- doi: 10.1371/journal.pmed.0040022 pmid: 17199408.
- Lewis JJ, Charalambous S, Day JH, Fielding KL, Grant AD, Hayes RJ, et al., et al. HIV infection does not affect active case finding of tuberculosis in South African gold miners. Am J Respir Crit Care Med 2009 doi: 10.1164/rccm.200806-846OC pmid: 19745207.
- Wood R, Middelkoop K, Myer L, Grant AD, Whitelaw A, Lawn SD, et al., et al. Undiagnosed tuberculosis in a community with high HIV prevalence: implications for tuberculosis control. Am J Respir Crit Care Med 2007; 175: 87-93 doi: 10.1164/rccm.200606-759OC pmid: 16973982.
- Mtei L, Matee M, Herfort O, Bakari M, Horsburgh CR, Waddell R, et al., et al. High rates of clinical and subclinical tuberculosis among HIV-infected ambulatory subjects in Tanzania. Clin Infect Dis 2005; 40: 1500-7 doi: 10.1086/429825 pmid: 15844073.
- Lawn SD, Myer L, Bekker LG, Wood R. Tuberculosis-associated immune reconstitution disease: incidence, risk factors and impact in an antiretroviral treatment service in South Africa. AIDS 2007; 21: 335-41 doi: 10.1097/QAD.0b013e328011efac pmid: 17255740.
- Gandhi NR, Moll A, Sturm AW, Pawinski R, Govender T, Lalloo U, et al., et al. Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa. Lancet 2006; 368: 1575-80 doi: 10.1016/S0140-6736(06)69573-1 pmid: 17084757.
- Isoniazid preventive therapy (IPT) for people living with HIV: Consensus statement of the Core Group of the TB/HIV Working Group of the StopTB Partnership. 2007. Available at: http://www.stoptb.org/wg/tb_hiv/assets/documents/IPT%20Consensus%20Statement%20TB%20HIV%20Core%20Group.pdf [Accessed on 29 October 2009]
- Corbett EL, Bandason T, Cheung YB, Makamure B, Dauya E, Matambo R, et al., et al. Prevalent infectious tuberculosis in the general population, Harare, Zimbabwe: burden, risk factors and implications for control. Int J Tuberc Lung Dis 2009; 13: 1231-7 pmid: 19793427.
- Babor TF, Higgins-Biddle JC, Saunders JB, Monteiro MG. The alcohol use disorders identification test (AUDIT). 2nd ed. Geneva: World Health Organization; 2001.
- Banerji D, Andersen S. A sociological study of awareness of symptoms among persons with pulmonary tuberculosis. Bull World Health Organ 1963; 29: 665-83 pmid: 14102040.
- El-Sony AI, Mustafa SA, Khamis AH, Sobhi S, Enarson DA, Baraka OZ, et al., et al. Symptoms in patients attending services for diagnosis of pulmonary tuberculosis in Sudan. Int J Tuberc Lung Dis 2003; 7: 550-5 pmid: 12797697.
- den Boon S, van Lill SW, Borgdorff MW, Enarson DA, Verver S, Bateman ED, et al., et al. High prevalence of tuberculosis in previously treated patients, Cape Town, South Africa. Emerg Infect Dis 2007; 13: 1189-94 pmid: 17953090.
- Banda HT, Harries AD, Welby S, Boeree MJ, Wirima JJ, Subramanyam VR, et al., et al. Prevalence of tuberculosis in TB suspects with short duration of cough. Trans R Soc Trop Med Hyg 1998; 92: 161-3 doi: 10.1016/S0035-9203(98)90727-1 pmid: 9764320.
- Swingler GH. Chest radiography in ambulatory children with acute lower respiratory infections: effective tuberculosis case-finding? Ann Trop Paediatr 2000; 20: 11-5 doi: 10.1080/02724930092002 pmid: 10824207.
- Scott JA, Hall AJ, Muyodi C, Lowe B, Ross M, Chohan B, et al., et al. Aetiology, outcome, and risk factors for mortality among adults with acute pneumonia in Kenya. Lancet 2000; 355: 1225-30 doi: 10.1016/S0140-6736(00)02089-4 pmid: 10770305.
- Nyamande K, Lalloo UG, John M. TB presenting as community-acquired pneumonia in a setting of high TB incidence and high HIV prevalence. Int J Tuberc Lung Dis 2007; 11: 1308-13 pmid: 18034951.
- Pai M, Kalantri S, Dheda K. New tools and emerging technologies for the diagnosis of tuberculosis: part II. Active tuberculosis and drug resistance. Expert Rev Mol Diagn 2006; 6: 423-32 doi: 10.1586/14737184.108.40.2063 pmid: 16706744.